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Item type: Publication , Access status: Metadata only , The mRNA covalent modification dihydrouridine regulates transcript turnover and photosynthetic capacity during plant abiotic stress(2026-06-25) Yu, Li’ang; Melandri, Giovanni; Dittrich, Anna C.Nelson; Calleja, Sebastian; Ganguly, Diep R.; Palos, Kyle; Brewer, Emily K.; Fischer, Hillary; Rozzi, Bruno; Srinivasan, Aparna; Obata, Toshihiro; AbdElgawad, Hamada; Beemster, Gerrit T.S.; Henderson, Riley; Garcia, Ciara; Zhang, Xiaodan; Stern, David; Eveland, Andrea; Lyons, Eric; Arnold, A. Elizabeth; Skirycz, Aleksandra; Schroeder, Susan J.; Gregory, Brian D.; Pauli, Duke; Nelson, Andrew D.L.RNA covalent modifications (RCMs) influence RNA stability and translation efficiency, and they thus play critical roles in eukaryotic growth and development. However, their role in regulating plant performance under abiotic stress remains largely unexplored. Here, we integrated multi-omics data in 6 Sorghum bicolor accessions under water-limiting conditions in the field to explore the relationship between RCMs and drought response. Within a stress- and photosynthesis-associated gene co-expression module, we identified SbDUS2, a member of a family of enzymes conserved across eukaryotes, that catalyzes the reduction of uracil to dihydrouridine (DHU) on RNA molecules. DHU-modified transcripts in this module were enriched for photosynthetic functions and showed strong correlation with photosynthetic traits. To elucidate the function of this RCM, we characterized loss-of-function dus2 mutants in Arabidopsis thaliana. Under control conditions, these DHU-deficient mutants exhibited impaired germination and delayed development. Furthermore, under water-limiting or heat conditions, these mutants showed significantly reduced net CO2 assimilation and survival. Using multiple transcriptome-wide RNA stability assays, we demonstrated that transcripts associated with lower DHU levels in a dus2 background generally exhibited increased stability compared to Col-0 controls. Particularly, lack of DUS2 led to the hyperstability of photosynthesis-related transcripts, impeding their turnover and likely preventing proper photosynthetic acclimation during stress. We propose a model where DHU acts as a critical post-transcriptional regulator marking mRNAs for rapid turnover under stress, highlighting an overlooked regulatory layer contributing to plant resilience.Item type: Publication , Access status: Metadata only , Information as a Cultural Category(Association for Computing Machinery (ACM), 2005) Dourish, Paul; Brewer, Johanna; Bell, GenevieveItem type: Publication , Access status: Open Access , ‘We campaign just by gathering 20 people’: suboptimal campaigning as a gendered campaign style in Medan, Indonesia(2026-02-01) Harahap, Aida; Jakimow, Tanya; Siahaan, Asima Yanty; YumasdaleniHow do we understand the gendered campaign styles of women candidates contesting elections in which they are structurally constrained with a low probability of success? We introduce the term ‘suboptimal campaigning’as a gendered campaign style to emphasise the accommodations women make due to broader gender orders that put them at an electoral disadvantage. Research with 24 female candidates in the 2024 local legislative elections in Medan, Indonesia, revealed their campaigns to be self-limiting in terms of where they campaign (spatial restrictions), who they target (predominantly women voters), when they campaign (temporal constraints) and what campaign practices they employ (limited financial investment and restricted activities). Rationalising female candidates’ decisions as strategically ‘running as a woman’obscures how Indonesia’s masculinised political institutions necessitate that women …Item type: Publication , Access status: Metadata only , A novel ALE scheme with the internal boundary for coupling tectonic and surface processes in geodynamic models(2026-06-30) Lu, Neng; Moresi, Louis; Giordani, Julian; Knight, Ben S.Recent advancements in modelling the deformation of the Earth's crust and upper mantle, coupled with surface processes, have significantly deepened our understanding of how the Earth responds to integrated tectonic and erosional forces, which are intricately linked to climate dynamics. This study presents a novel coupling framework within the Arbitrary Lagrangian–Eulerian with Internal Boundary (ALE-IB) scheme, which integrates the geodynamic codes Underworld 2 with the surface process code Badlands. Our innovative approach addresses the limitations of previous Eulerian-based coupling frameworks by maintaining the integrity of internal interfaces and providing precise surface tracking. This ensures accurate representation of material boundaries and enhances the fidelity of simulations involving complex geological processes. We detail the principles underlying the coupling of surface processes with tectonic deformation, leveraging the strengths of the ALE-IB scheme to model free surfaces and moving boundaries effectively. By comparing our model's performance with an Eulerian-based approach, we highlight key differences in structural and dynamic behaviour under varying surface process intensities. This comparison offers valuable insights into the intricate interactions between surface and deep Earth processes. Our findings contribute to a more comprehensive understanding of geomorphological and tectonic evolution, providing a robust framework for future research in geodynamic and climate-related geological studies.Item type: Publication , Access status: Metadata only , Socioeconomic and Geographical Analysis on Antenatal Care Visits in Indonesia: A Cross-sectional Study(2026) Rachmawati, Tety; Supriyanto, Stefanus; Musadad, Dede Anwar; Notobroto, Hari Basuki; Fitrianti, Yunita; Pawitaningtyas, Indah; Nugroho, Arief Priyo; Handayani, Sri; Khaqiqi, Nantabah Zainul; Effendi, Diyan ErmawanBackground: Antenatal care (ANC) is crucial for reducing maternal and neonatal mortality by detecting and managing pregnancy complications. In Indonesia, a policy mandating at least six ANC visits (K6) per pregnancy has been introduced, yet factors influencing adequate ANC under this new policy remain unevaluated. This study examines the national and sub-national prevalence of adequate ANC visits as well as its associated geographic and socioeconomic factors. Methods: This cross-sectional study analyzed data from the 2023 Indonesian Health Survey, which included 70,916 women of reproductive age. Logistic regression was used to assess predictors of ANC adequacy, such as age, education, marital status, health insurance, employment, urbanicity, economic status, and travel time to healthcare facilities. The data were analyzed through SPSS version 23 using descriptive statistics, chi square test, and multiple logistic regression. Final regression model was based on significant predictors only with calculated adjusted odd ratios and 95% confidence interval (CI) with a significance level of 0.05. Results: The national prevalence of adequate ANC visits was 38.65%. However, a significant disparity was observed between provinces, with eastern areas showing much lower prevalence than provinces in western Indonesia. ANC adequacy was highest among women aged 25–29 and lowest among those under 20. Higher education (aOR=1.45, P<0.001), combined health insurance (aOR=2.84, P<0.001), employment (aOR=1.07, P<0.001), urban residence (aOR=1.24, P<0.001), and higher economic status (aOR=1.60, P<0.001) were positively associated with ANC adequacy. Conversely, being divorced (aOR=0.76, P<0.001) and widowed (aOR=0.74, P=0.004), and longer travel times to primary healthcare center negatively affected compliance (aOR=0.68, P<0.001). Conclusion: Addressing geographic and socioeconomic barriers through improved healthcare infrastructure, transportation support, and expanded insurance coverage is essential for increasing ANC utilization and improving maternal health in Indonesia.